Pt K1 (NBL-3)

Origin: rat kangaroo, kidney.

            Nature 1962. 194: 406; Cytogenetics 1964. 3: 19.; Cytology (Russ) 1988.30: 732-738;

Cytology  (Russ)  1996. 38: 75-84..

Morphology: epithelial-like  

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM

serum -   FBS 10%

other components -   NEAA 1%, sodium pyruvate 1mM (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:2 - 1:3, optimal population density 4.0-5.0104 cells/cm2

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 12, variability in the range between 10-17 chromosomes,

 modal number of chromosomes 11 without markers, one small metacentric

of the diploid female karyotype is absent , number of polyploid cells 2%.

Plating efficiency:    2% (ATCC)

Other properties:

virus susceptibility: vesicular stomatitis (Indiana)

Applications:  cell biology, cytogenetics, virology.

Collections: ATCC CCL 35; ECACC 91013163; MWIIW; SPBIC.