PSP

Origin: pig, kidney.

Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 0.5% hemohydrolyzate or BME/LAH (1:1).

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:5.

cryoconservation - culture medium 50%, BS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Karyology:   2n= 38, modal number of chromosomes 34.

Other properties:

virus susceptibility: corona- and entoroviruses of swine.

Applications: virology, biotechnology.

Collections: MWIEV.