Psi 2 BAG
mouse NIH/Swiss, embryo.
Proc.Natl.Acad.Sci. 1987. 84: 156-160; Nature 1987. 328: 131-136.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
97% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 40, variability in the range between 63-74 chromosomes,
modal number of chromosomes 70, number of markers - 1 telocentric chromosome
with secondary constriction (routine dye), 1 microchromosome, number of polyploid cells 1.5%.
this line produces a vector (BAG) that can infect mouse and rat and transduce the bacterial
b galactosidase gene.
Collections: ATCC CRL 9560; SPBIC.