Psi 2 BAG a

Origin: mouse NIH/Swiss, embryo.

            Proc.Natl.Acad.Sci. 1987. 84: 156-160; Nature 1987. 328: 131-136.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:4

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     97% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 63-74 chromosomes,

modal number of chromosomes 70, number of markers - 1 telocentric chromosome

with secondary constriction (routine dye), 1 microchromosome, number of polyploid cells 1.5%.

Other properties:

this line produces a vector (BAG) that can infect mouse and rat and transduce the bacterial b galactosidase gene.

Applications: genetical transformation.

Collections: ATCC CRL 9560; SPBIC.