PSGK-S-85

Origin:   Asiatic ibex, kidney.

Abstract, Meeting « Virus Diseases of Domestic Animals», 17-21.04.1995. 22:82.  Vladimir.

Morphology: epithelial-like

Mode of cultivation: suspension

Conditions for cultivation: medium -  0.25% EMPH-d on solution of Earle

serum -  BS 10%

other components -  glutamine 0.06%, glucose 4.5 g/l

subculture procedure - supporting method of taking away and

adding in the stir suspension, split ratio  1:4-1:6.

cryoconservation - culture medium 45%, BS 45%, DMSO 10%,  5.0ő106 cells/ml in ampule

Viability after cryoconservation:   80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Karyology:   modal number of chromosomes 60-61.

Other properties:

virus susceptibility: TDV; hog cholera virus; ovine contagious fever virus; hemorrhagic disease of deer.

Presence of oncornavirus C

Applications: virology , biotechnology

Collections: MWIEV