PSGK-60

Origin: Asiatic ibex, kidney.

            Abstracts, Meeting «Virus Diseases of Domestic Animals», Vladimir, 1995: 22.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 0.25% EMPH-d

serum -   BS 10%

other components -   50 mg/ml oxytetracycline hydrochloride

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:10), split ratio 1:6.

cryoconservation - 0.25% EMPH-d 70%, BS 20%, DMSO 10%, 5.0ő106 cells/ml in ampule

Viability after cryoconservation:     80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   modal number of chromosomes 60-62.

Other properties:

virus susceptibility: different taxonomic groups.

Presence of oncornavirus C.

Applications: virology, biotechnology.

Collections: MWIEV