Virology 1962. 16: 205.
Mode of cultivation:
Conditions for cultivation:
medium - 199
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:5, optimal population density
cryoconservation - medium - 199 80%, FBS 20%, glycerol 10%, 3.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation:
72% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 38, variability in the range between 52-56 chromosomes,
modal number of chromosomes 54-55, without markers (routine dye), number of polyploid cells 10.0%.
virus susceptibility: reoviruses 1-3; adenovirus 12; Japanese encephalitis virus and other arboviruses.