PS

Origin: pig, kidney.

            Virology 1962. 16: 205.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:5, optimal population density 0.5-1.0105 cells/cm2

cryoconservation - medium - 199 80%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     72% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 38, variability in the range between 52-56 chromosomes,

modal number of chromosomes 54-55, without markers (routine dye), number of polyploid cells 10.0%.

Other properties:

virus susceptibility: reoviruses 1-3; adenovirus 12; Japanese encephalitis virus and other arboviruses.

Applications: virology.

Collections: MWIIW.