PS-FGM

Origin: saiga, kydney.

Abstracts, the 3-d Meeting « Cultivation of Human and Animals Cells», 1990: 99.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  EMPH-d

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3  

cryoconservation - EMPH-d 60%,  BS 30%, DMSO 10%, 2.0-3.0x106 cells/ml in ampule

Viability after cryoconservation:   85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:  modal number of chromosomes  48.

Other properties:

virus susceptibility: bovine diarrhea virus; vesicular stomatitis; bovine and equine small pox viruses.

Applications: virology, biotechnology 

Collections: MWIEV