pig, kidney, clone of cell line RS-88.
In « Foot and Mouth Disease», Vladimir, 1991.
epithelial-like (in monolayer)
Mode of cultivation:
Conditions for cultivation: medium - BME on Earle’s solution - for monolayer cultivation;
BME on Earle’s solution with 10x NaH2PO4 without Ca+; 0.25% hemohydrolyzate;
g/l glucose; 0.15 g/l glutamine; 0.05% yeast extract; pH 7.2-7.5 - for
serum - FBS 5%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split
cryoconservation - culture medium 45%, BS 45%, DMSO 10%, 2.0-3.0ơ106 cells/ml in ampule
Viability after cryoconservation:
78% (0 passage, dye trypan blue)
tests for bacteria, fungi were negative
karyological and isoenzymological (LDH, G6PD) analysis
2n= 38, modal number of chromosomes 54-60.
virus susceptibility: Gamboro; mink enteritis; swine diseases viruses.
Production of Gamboro; egg drop syndrom and mink enteritis viruses.