PPES

Origin: pig, kidney.

            Veterinary (Russ.) 1975. 9: 45-47; Bull.of VIEV, issue 49. 1983: 64-68.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - LAH/BME (2:1)

serum -  BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3

cryoconservation - culture medium 50%, BS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Other properties:

virus susceptibility: hog cholera, enterovirus and transmissible gastroenteritis of swine.

Applications: virology, biotechnology.

Collections: MWIEV.