PPEO

Origin: sheep, embryo , kidney

Inf. Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  199 or LAH or BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:5), split ratio 1:2-1:3.

cryoconservation - culture medium70%, BS 20%, glycerol 10%,  2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:   80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n=54,  variability in the range between 24-85 chromosomes,

modal number of chromosomes 45.

Other properties:

virus susceptibility: toga and herpes viruses.

Applications: virology, biotechnology

Collections: MWIEV