PO-88

Origin: rhesus, monkey, kidney.

Submitted by Glinskikh N.P. et al., EVIRI, 1988.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/EMEM (1:1)

serum -   BS 5-7%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:3-1:4, optimal population density 1.3 -1.5x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.0 -1.5 106 cells/ml in ampule

Viability after cryoconservation:   80 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 40-69  chromosomes,

modal number of chromosomes 42. 

Plating efficiency:  70%

Other properties:

virus susceptibility: TBE; parainfluenza 2,3; human adenovirus 7; enteroviruses.

Applications: virology.

Collections: ESCC