rhesus, monkey, kidney.
Submitted by Glinskikh N.P. et al., EVIRI, 1988.
Mode of cultivation:
Conditions for cultivation:
medium - 199/EMEM (1:1)
serum - BS 5-7%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:3-1:4, optimal population density 1.3 -1.5x105
cryoconservation - growth medium, 10% glycerol, 1.0 -1.5
õ106 cells/ml in ampule
Viability after cryoconservation:
80 % (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 42, variability in the range between 40-69 chromosomes,
modal number of chromosomes 42.
virus susceptibility: TBE; parainfluenza 2,3; human adenovirus 7; enteroviruses.