P- x C

Origin: hybrid cells - sheep kidney -50- x sheep splenocytes.-TxC

Submitted by I.L.Kulikova,T.V.Galnbek, L.P.Dyakonov et al., 1999.

Morphology: epithelium- and lymphocyte-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

subculture procedure - harvesting of the cells in suspension is carried out by deposition,

detach from flask using trypsin 0,25% : EDTA 0,02% (1:9); split ratio 1:3-1:4.

cryoconservation - EMEM 50%, FBS 40%, DMSO10%, 2-3x10 6 cells/ml in ampule

Viability after cryoconservation: 80-85% (0 passage, dye trypan blue)

Sterility: t ests for bacteria,fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=54, variability in the range between 38-58 chromosomes;

modal number of chromosomes 54.

Other properties:

virus susceptibility: IBR, herpes virus, virus rhinopneumonia of equine type 1

Applications: cell biotechnology, virology, biotechnology.

Collections: MWIEV