PÎ-ÒÊ x C
Origin:hybrid cells - sheep kidney ÏÎ-50-ÒÊ x sheep splenocytes.ÏÎ-TÊxC
Submitted by I.L.Kulikova,T.V.Galnbek, L.P.Dyakonov et al., 1999.
Morphology: epithelium- and lymphocyte-like
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - FBS 10%
subculture procedure - harvesting of the cells in suspension is carried out by deposition,
detach from flask using trypsin 0,25% : EDTA 0,02% (1:9); split ratio 1:3-1:4.
cryoconservation - EMEM 50%, FBS 40%, DMSO10%, 2-3x10 6 cells/ml in ampule
Viability after cryoconservation: 80-85% (0 passage, dye trypan blue)
Sterility: t ests for bacteria,fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n=54, variability in the range between 38-58 chromosomes;
modal number of chromosomes 54.
virus susceptibility: IBR, herpes virus, virus rhinopneumonia of equine type 1
Applications: cell biotechnology, virology, biotechnology.