Bulletin WIEV. 1972. 14: 67.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:2-1:3, optimal population density
cryoconservation - BME 80%, FBS 20%, glycerol 10%, 3.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation:
70-85% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 54, variability in the range between 40-55 chromosomes,
modal number of chromosomes 52, the cells have microchromosomes, number of
polyploid cells 18.0%.
virus susceptibility: rhynotracheitis; parainfluenza 3; vaccine strain LG of bovine plague virus;
foot and mouth disease; herpes; Newcastle disease; Aujeszky strain pseudorabies virus.