PO-2

Origin:  sheep, kydney.

Bulletin WIEV. 1972. 14: 67.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  BME

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:2-1:3, optimal population density 1.0105 cells/ml

cryoconservation - BME 80%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     70-85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 54, variability in the range between 40-55 chromosomes,

modal number of chromosomes 52, the cells have microchromosomes, number of polyploid cells 18.0%.

Other properties:

virus susceptibility: rhynotracheitis; parainfluenza 3; vaccine strain LG of bovine plague virus;

foot and mouth disease; herpes; Newcastle disease; Aujeszky strain pseudorabies virus.

Applications: virology, veterinary.

Collections: MWIIW.