PNK-86

Origin: rabbit, new-born kidney

Submitted by Kolesnikova G.G. et al., EVIRI, patent N 1613487, 1.06.88

Morphology:    epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/EMEM (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:3-1:4, optimal population density 1.0x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 2.0 106 cells/ml in ampule

Viability after cryoconservation: 70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 44, variability in the range between 41-85  chromosomes,

modal number of chromosomes 66. 

Plating efficiency:  70% (ESCC)

Other properties:

virus susceptibility: TBE; parainfluenza 2,3; human adenovirus 7; enteroviruses.

Applications: virology.

Collections: ESCC, MWIEV.