rabbit, new-born kidney
Submitted by Kolesnikova G.G. et al., EVIRI, patent N 1613487, 1.06.88
Mode of cultivation:
Conditions for cultivation:
medium - 199/EMEM (1:1)
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:3-1:4, optimal population density 1.0x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 2.0
õ106 cells/ml in ampule
Viability after cryoconservation:
70% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 44, variability in the range between 41-85 chromosomes,
modal number of chromosomes 66.
virus susceptibility: TBE; parainfluenza 2,3; human adenovirus 7; enteroviruses.
Collections: ESCC, MWIEV.