PKK-FGM-10

Origin: dwarf goat, kidney.

            Kalugina I.A. PhD thesis, Moscow 1992.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME with addition 0.06% EMPH-d

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:9), split ratio 1:2-1:4

cryoconservation - culture medium 50%, BS 40%, DMSO 10%, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:     79% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Other properties:

chlamidia susceptibility:

Applications: virology, biotechnology.

Collections: MWIEV