dwarf goat, kidney.
Kalugina I.A. PhD thesis, Moscow 1992.
Mode of cultivation:
Conditions for cultivation:
medium - BME with addition 0.06% EMPH-d
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split
cryoconservation - culture medium 50%, BS 40%, DMSO 10%, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation:
79% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative