PK-82

Origin: rabbit, kydney.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  BME

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density  1.0x105  cells/ml

cryoconservation - BME 70%, BS 20%, glycerol 10%, 5.0 -10.0106 cells/ml in ampule

Viability after cryoconservation:   90 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDG, G6PD) analysis

Karyology:   2n=44, variability in the range between 38-45 chromosomes,

modal number of chromosomes 44, number of markers -1, large submetacentric

chromosome (routine dye), number of polyploid cells 8.0%.

Other properties:

virus susceptibility: herpes simplex; Newcastle disease; Aujesky; foot-and-mouth disease;

bovine rhynotracheitis; parainfluenza.

Applications: virology

Collections: MWIIW.