Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density
cryoconservation - BME 70%, BS 20%, glycerol 10%, 5.0 -10.0õ106 cells/ml in ampule
Viability after cryoconservation:
90 % (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDG, G6PD) analysis
Karyology: 2n=44, variability in the range between 38-45 chromosomes,
modal number of chromosomes 44, number of markers -1, large submetacentric
chromosome (routine dye), number of polyploid cells 8.0%.
virus susceptibility: herpes simplex; Newcastle disease; Aujesky; foot-and-mouth disease;
bovine rhynotracheitis; parainfluenza.