PK(15)

Origin: pig, kidney.

            Am.J.Vet.Res. 1968. 29: 153; J.Genet.Virol. 1971. 10; 195-198; Vet.microbiol. 1982. 7: 515.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -   FBS 5-10%

other components -   NEAA 1%, sodium pyruvate 1mM

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:5, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 38, variability in the range between 30-38 chromosomes,

modal number of chromosomes 37, number of markers - 1 (routine dye),

number of polyploid cells 5%.(ATCC)

Plating efficiency:    2% (ATCC)

Other properties:

virus susceptibility: vesicular stomatitis (Indiana); vaccinia; reovirus 2, 3; adenovirus 4, 5;

Coxsackie B-2, B-3, B-4, B-5, B-6; pseudorabies; swine fever virus, swine pestis virus

Applications: virology.

Collections: ATCC CCL 33; ECACC 85022110; SPBIC.