PES

Origin: pig, embryo, kidney.

            Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.

Morphology: epithelial-like and fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - LAH/BME (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2.

cryoconservation - culture medium, BS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     72% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 38, diploid

Other properties:

virus susceptibility: swine parvo-, rota-, coronaviruses.

Applications: virology, cell biology.

Collections: MWIEV