PEO

Origin: sheep, embryo, kidney.

Submitted by  Glinskikh N.P. et al., EVIRI, Patent N 1364403. 8. 01. 85.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/EMEM (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:3-1:4, optimal population density 1.3 -1.5x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.5-2.0 106 cells/ml in ampule

Viability after cryoconservation:   90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=54, variability in the range between 41-57 chromosomes,

modal number of chromosomes 54 

Plating efficiency:  85 %

Other properties:

virus susceptibility: TBE; parainfluenza; Coxsackie B.

Finite lifetime culture.

Applications: virology.

Collections: ESCC