sheep, embryo, kidney.
Submitted by Glinskikh N.P. et al., EVIRI, Patent N 1364403. 8. 01. 85.
Mode of cultivation:
Conditions for cultivation:
medium - 199/EMEM (1:1)
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:3-1:4, optimal population density 1.3 -1.5x105
cryoconservation - growth medium, 10% glycerol, 1.5-2.0
õ106 cells/ml in ampule
Viability after cryoconservation:
90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=54, variability in the range between 41-57 chromosomes,
modal number of chromosomes 54
virus susceptibility: TBE; parainfluenza; Coxsackie B.
Finite lifetime culture.