Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.
epithelial-like and fibroblast-like
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split
cryoconservation - BME 50%, FBS 40%, DMSO 10%, 2.0-3.0õ106 cells/ml in ampule
Viability after cryoconservation:
70% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
virus susceptibility: IBR, BVD.
cell biology, virology.