mouse strain 129, testicular teratocarcinoma.
Exp.Cell Res. 1979. 120: 1.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:2), do not
allow cultures to become completely confluent.
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
deficient in hypoxanthine phosphoribosyl transferase
Resistant to 8-azaguanine and 6-thioguanine