PA 317

Origin: mouse, embryo. This line was derived from NIH/3T3 TK- cells by cotransfection

with retrovirus packaging construct DNA (pPAM3) and the herpes simplex virus thymidine kinase (TK) gene.

            Mol.Cell Biol. 1986. 6: 2895-2902; N.Engl.J.Med. 1990. 232: 570-578.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:3), split ratio 1:2 - 1:3, optimal population density 3.0-5.0x104  cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Applications: genetical transformation, virology.

Collections: ATCC CRL 9078; ECACC 89032007; SPBIC.