Origin: mouse, embryo. This line was derived from NIH/3T3 TK- cells by cotransfection
with retrovirus packaging construct DNA (pPAM3)
and the herpes simplex virus thymidine kinase (TK) gene.
Mol.Cell Biol. 1986. 6: 2895-2902; N.Engl.J.Med. 1990. 232: 570-578.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split
ratio 1:2 - 1:3, optimal population density 3.0-5.0x104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
genetical transformation, virology.
ATCC CRL 9078; ECACC 89032007; SPBIC.