P3X63Ag8.653

Origin: mouse BALB/c, myeloma, clone of P3X63Ag8.

            J.Immunol. 1979. 123: 1548.

Morphology: lymphoid

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum -   FBS 10-20%

subculture procedure  optimal population density 3.0-5.0x105 cells/ml

cryoconservation - growth medium, 10% DMSO, 5.0-6.0106 cells/ml in ampule

Viability after cryoconservation:     71% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 40, variability in the range between 46-61 chromosomes,

 modal number of chromosomes 51-53, number of markers - 1-3 meta- and

 submetacentric chromosomes (routine dye), number of polyploid cells 2%.

Other properties:

does not secrete Ig.

Resistant to 8- azaguanine

Applications: fusion partner for hybridomas, tumorigenicity.

Collections: ATCC CRL 1580; ECACC 85011420; DSM ACC 43; MWIIW; SPBIC.