Origin: mouse BALB/c, myeloma, clone of P3X63Ag8.

            Exp. Cell Res. 1970. 60:61; J. Mol. Biol. 1974. 90: 691; Eur. J. Immunol. 1976. 6: 511.

Morphology: lymphoid

Mode of cultivation: suspension

Conditions for cultivation: medium - DMEM/F12

serum -   FBS 10%

subculture procedure optimal population density 1.0-5.0105 cells/ml

cryoconservation - growth medium, 10% DMSO, 5.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Other properties:

does not secrete Ig.

Resistant to 8-azaguanine

Applications: fusion partner for hybridomas, tumorigenicity.

Collections: ATCC TIB 18; DSM ACC 145; ECACC 85011427; MWIIW; SPBIC.