P388D1, clone P2

Origin: DBA/2 mouse, lymphoid neoplasma, induced by methylcholantrene.

Amer. J. Path. 1957, 33:603; J. Immunol. 1978, 120: 1497; J. Immunol. 1987, 139: 780.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium -  RPMI 1640

serum - BS 10 %

other components -   sodium pyruvate 1 mM

subculture procedure - optimal cell density 5.0x105 cells/ml

cryoconservation -  95 % BS, 5 % DMSO,  1.5 - 2.0x106 cells/ml in ampule.

Viability after cryoconservation: 70 - 80 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species:   karyological analysis

Other properties:

production of interleukines 1 and 6 under the lipopolysaccharide stimulation.

Applications: cell biology

Collections: SPBII.