mouse DBA/2, lymphoid neoplasm induced by methylcholanthrene.
Am.J.Pathol. 1957. 33: 603.
Mode of cultivation:
Conditions for cultivation:
medium - RPMI 1640
serum - FBS 10%
- optimal population density 1.0x105 cells/ml
cryoconservation - growth medium, 10% DMSO, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation:
80% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 40, variability in the range between 38-44 chromosomes,
modal number of chromosomes 41-42, number of markers - 6 (differential dye, ATCC),
the most cells have 3-5 microchromosomes including double minute chromosomes, number of polyploid cells 4.5%.
the cells cannot be plated (ATCC)
tumorigenic in nude mice
cell biology, tumorigenicity.
Collections: ATCC CCL 46; SPBIC.