P388 D1

Origin: mouse DBA/2, lymphoid neoplasm induced by methylcholanthrene.

            Am.J.Pathol. 1957. 33: 603.

Morphology:  lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum -   FBS 10%

subculture procedure - optimal population density 1.0x105  cells/ml

cryoconservation - growth medium,  10% DMSO, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 38-44 chromosomes,

modal number of chromosomes 41-42, number of markers - 6 (differential dye, ATCC),

the most cells have 3-5 microchromosomes including double minute chromosomes, number of polyploid cells 4.5%.

Plating efficiency:  the cells cannot be plated (ATCC)

Tumorigenicity:  tumorigenic in nude mice

Applications: cell biology, tumorigenicity.

Collections: ATCC CCL 46; SPBIC.