mouse C3H/He, teratocarcinoma.
Dev. Biol. 1982. 89: 503-508; J. Cell Biol. 1982. 94: 253-262;
Nature 1982. 299: 165-167.
Mode of cultivation:
Conditions for cultivation:
serum - FBS 2.5%, CS 7.5% or FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split
cryoconservation - growth medium, 5%DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
75% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
2n=40, normal mouse karyotype (40, XY).
high efficiency in medium containing 10-4Μ
Can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid;
in the presence of DMSO differentiate into cardiac and skeletal muscle.
Collections: ATCC CRL 1825; SPBIC.