P19

Origin: mouse C3H/He, teratocarcinoma.

            Dev. Biol. 1982. 89: 503-508; J. Cell Biol. 1982. 94: 253-262;

Nature 1982. 299: 165-167.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - aΜΕΜ

serum -   FBS 2.5%, CS 7.5% or FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:2

cryoconservation - growth medium, 5%DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=40, normal mouse karyotype (40, XY).

Plating efficiency:   high efficiency in medium containing 10-4Μ b-mercaptoethanol (ATCC).

Other properties:

Can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid;

in the presence of DMSO differentiate into cardiac and skeletal muscle.

Applications: differentiation.

Collections: ATCC CRL 1825; SPBIC.