P-815

Origin: mouse DBA/2, mastocytoma induced by methylcholanthrene.

            J.Natl.Cancer Inst. 1957. 18: 587; Cell Immunol. 1973. 9: 60; J.Immunol. 1973. 111: 389;

J.Immunol. 1977. 119: 950; Nature 1974. 249: 49;

Biochem.Biophys.Res.Commun. 1974. 61: 1268; Cancer Res. 1977. 37: 546.

Morphology:  round cells

Mode of cultivation: suspension

Conditions for cultivation: medium - DMEM or DMEM/F12

serum -   FBS 10%

subculture procedure - optimal population density 3.0-9.0105  cells/ml

cryoconservation - growth medium,  10% DMSO, 5.0106 cells/ml in ampule

Viability after cryoconservation:     70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Other properties:

lysozyme synthesis

Applications:  target cell for cytotoxic T-cell assays, immunology, cell biology.

Collections: ATCC TIB 64; DSM ACC1; SPBIC.