NSO/1

Origin: mouse, clone of myeloma P3X63Ag8.

            Methods Enzymol. 1981. 73B: 3.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640 or DMEM/F12

serum -   FBS 10%

subculture procedure  - optimal population density 5.0-9.0105 cells/ml

cryoconservation - growth medium, 10% DMSO, 5.0-6.0106 cells/ml in ampule

Viability after cryoconservation:     85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 40-65 chromosomes,

modal number of chromosomes 60, number of markers - 2-5 meta- and

submetacentric chromosomes (routine dye), number of polyploid cells 2.8%.

Other properties:

does not synthesize Ig.

Resistant to 8-azaguanine

Applications:  fusion partner for hybridomas.

Collections: MWIIW; SPBIC.