NRK-49F

Origin: rat, kidney.

            J.Cell Physiol. 1978. 94: 35-342.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F10 or F12

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3 - 1:6

cryoconservation - growth medium,  10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 37-43 chromosomes,

modal number of chromosomes 40, number of markers - 1 (routine dye),

some cells have 1-2 dicentrics and 1-4 microchromosomes, number of polyploid cells 14%.

Other properties :

virus susceptibility: murine sarcoma virus.

EGF receptors.

Applications: genetical transformation, cell biology.

Collections: ATCC CRL 1570; ECACC 86101301; SPBIC.