NGUK-1

Origin: rat, Gasser node neurinoma.

            Actual Problems of Modern Hystopathology, Moscow. 1983: 71;

Vestnik AMS USSR (Russ.) 1984. 1: 36; Cytology (Russ.) 1989. 31: 97-101;

Voprosy Virusol. (Russ.) 1997. 5: 203.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -   FBS 10-20%

other components -   chicken embryo extract 5% (MWIIW).

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:3-1:4

cryoconservation - growth medium, DMSO or glycerol 10%,  3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 42, variability in the range between 39-45 chromosomes,

modal number of chromosomes 41, number of polyploid cells 26.0%.

Tumorigenicity:  tumorigenic in newborn rats.

Other properties:

virus susceptibility: vesicular stomatitis, rabbies, TBE virus, Synabis,

hog cholera virus, bovine plague, Coxsackie A7.

Isoenzymes LDH and G6PD.

Persistention of transmissive spongioencephalopaties exciters, in particular

creutzfeld Jakob disease, Hersmann-Schtreussler syndrom and skrepie agent.

Applications: virology, oncology, cell biology, biotechnology.

Collections: MWIEV, MWIIW.