Neuro-2a

Origin: mouse A (albino), neuroblastoma.

            J.Cell Biol. 1969. 43: 69A; Proc.Natl.Acad.Sci. 1970. 65: 129-136.

Morphology:  neuron-like and amoeboid-like.

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -   FBS 10%

other components -   NEAA 1%

subculture procedure - cells detach from flask using EDTA 0.02% (1:3),

split ratio 1:2 - 1:5, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 5%DMSO, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     91% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH and G6PD) and immunofluorescent analysis

Karyology:   2n= 40, variability in the range between 70-96 chromosomes without modal

number, 32% of cells have middle metacentric chromosome with gap (routine dye),

each cell have 1-7 microchromosomes.

Plating efficiency:    60% (SPBIC)

Tumorigenicity:  tumorigenic in syngeneic animals.

Other properties:

Virus susceptibility: vesicular stomatitis (Indiana), herpes simplex.

Microtubular protein synthesis

Applications: differentiation, tumorigenicity, neurophysiology, cytoskelet study.

Collections:   ATCC CCL 131; ECACC 89121404; SPBIC.