NCTC clone 929 (ESCC)

Origin: mouse C3H/An, connective, subline of NCTC clone 929.

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/BME (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:3 - 1:5, optimal population density 1.0-1.3105 cells/cm2

cryoconservation - growth medium, glycerol 10%, 1.0106 cells/ml in ampule

Viability after cryoconservation:     75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 35-56 chromosomes,

modal number of chromosomes 45-55.

Plating efficiency:    70%

Other properties:

virus susceptibility: arboviruses.

Presence of oncoviruses A, C.

Applications: virology.

Collections: ESCC.