NCTC clone 929 (ESCC)
mouse C3H/An, connective, subline of NCTC clone 929.
Mode of cultivation:
Conditions for cultivation:
medium - 199/BME (1:1)
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:3 - 1:5, optimal population density 1.0-1.3õ105 cells/cm2
cryoconservation - growth medium, glycerol 10%, 1.0õ106 cells/ml in ampule
Viability after cryoconservation:
75% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological, isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 40, variability in the range between 35-56 chromosomes,
modal number of chromosomes 45-55.
virus susceptibility: arboviruses.
Presence of oncoviruses A, C.