NCTC clone 929

Origin: mouse C3H/An, connective, clone of cell line L.

            J.Natl.Cancer Inst. 1943. 4: 165; J.Natl.Cancer Inst. 1948. 9: 229;

J.Natl.Cancer Inst. 1951. 12: 133; 1953. 14: 655; Cancer Res. 1956. 16: 162;

J.Biophys.Biochem.Cytol.1958. 4: 567; Natl.Cancer Inst.Monogr.1962. 7: 147.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -   FBS or BS 10%

other components -   NEAA 1%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:3 - 1:10, optimal population density 1.0-3.0104 cells/cm2

cryoconservation - growth medium, 5-10% DMSO, 1.0-1.5106 cells/ml in ampule

Viability after cryoconservation:     80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH and G6PD) and immunofluorescent analysis

Karyology:   2n= 40, variability in the range between 58-66 chromosomes,

modal number of chromosomes 63-64, number of markers - 36 including 1

polycentric (differential dye), number of polyploid cells 1%.

Plating efficiency:    40% (SPBIC)

Tumorigenicity:  tumorigenic in syngeneic animals

Other properties:

virus susceptibility: pseudorabies, vesicular stomatitis, paramixovirus, togaviruses, herpes simplex.

Susceptibility to chlamidia

Applications: tumorigenicity, differentiation, virology, biotechnology.

Collections: ATCC CCL 1; ECACC 88102702; MWIIW; SPBIC; SPBII.