NB41A3

Origin: mouse A, neuroblastoma, clone of C1300.

            Proc.Natl.Acad.Sci. 1962. 48: 1184-1190.

Morphology:  neuroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F10

serum -   HS 12.5%, FBS 2.5%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:2 - 1:4, optimal population density 3.0-5.0x104  cells/cm2

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and immunofluorescent analysis

Karyology:   2n= 40, variability in the range between 67-99 chromosomes without

modal number, number of markers - 6-10 metacentrics (routine dye).

Plating efficiency:    80% (SPBIC)

Other properties:

virus susceptibility: vesicular stomatitis, herpes simplex, vaccinia.

Acetylcholinesterase, choline acetylase and tyrosine hydroxylase production.

Applications:  tumorigenicity, enzymology, virology, differentiation.

Collections: ATCC CCL 147; ECACC 89121405; SPBIC.