Mv 1 Lu (NBL-7)

Origin: mink, lung.

            Virology 1974. 60: 282-287.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM

serum -   FBS 10%

other components -   NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:2 - 1:4, optimal population density 2.0-4.0104  cells/cm2

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyologiical analysis

Karyology:   2n= 30, variability in the range between 24-32 chromosomes,

modal number of chromosomes 30, pseudodiploid, number of markers - 1

dicentric in some cells (routine dye) (ATCC).

Plating efficiency:    5% (ATCC)

Other properties:

virus susceptibility: herpes simplex; reovirus 3; vesicular stomatitis; vaccinia; pseudorabies;

IBR; murine sarcoma virus, feline sarcoma virus.

Applications:  virology.

Collections: ATCC CCL 64; ECACC 88050503; MWIIW; SPBIC.