MH-22a

Origin: mouse C3HA, hepatoma.

            Bull.Exp.Biol.Med. Russ. 1972. 5: 94-95.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:2), split ratio 1:5 - 1:10

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH and G6PD) and immunofluorescent analysis

Karyology:   2n= 40, variability in the range between 50-60 chromosomes,

modal number of chromosomes 55, number of markers - 2 large and middle

submetacentric chromosomes, some cells have middle telocentric chromosome

with secondary constriction (routine dye).

Tumorigenicity:  tumorigenic in syngeneic animals

Other properties:

virus susceptibility: adenovirus 6.

Transferrin synthesis

Applications:  tumorigenicity, cell biology.

Collections: SPBIC.