mouse C3HA, hepatoma.
Bull.Exp.Biol.Med. Russ. 1972. 5: 94-95.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:2), split
ratio 1:5 - 1:10
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
98% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological, isoenzymological (LDH and G6PD) and immunofluorescent analysis
Karyology: 2n= 40, variability in the range between 50-60 chromosomes,
modal number of chromosomes 55, number of markers - 2 large and middle
submetacentric chromosomes, some cells have middle telocentric chromosome
with secondary constriction (routine dye).
tumorigenic in syngeneic animals
virus susceptibility: adenovirus 6.
tumorigenicity, cell biology.