MDCK (NBL-2)

Origin:  dog, kidney.

            Proc.Soc.Exp.Biol.Med. 1958. 98: 574.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM, BME or DMEM

serum -   FBS 10%

other components -   NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:3), split ratio 1:3 - 1:10 , optimal population density 1.0-3.0104 cells/cm2

cryoconservation - growth medium (may add 30% FBS), 10% DMSO, 1.0106 cells/ml in ampule

Viability after cryoconservation:    90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 78, variability in the range between 75-83  chromosomes,

modal number of chromosomes 78-80, number of markers - 1-2 large submetacentric

chromosomes, some cells have 1-2 middle meta- or submetacentric

chromosomes (routine dye), number of polyploid cells 0.6%.

Plating efficiency:    35% (ATCC)

Other properties:

virus susceptibility: vesicular stomatitis, vaccinia, Coxsackie B-5, reovirus 2, 3;

adenovirus 4, 5; influenza A, B, C; carnivorous plague, arboviruses, arenaviruses, i

nfectious canine hepatitis, swine vesicular exanthema.

Applications:  virology, biotechnology, cell biology.

Collections: ATCC CCL 34; ECACC 84121903; 85011435; MWIIW; ESCC; SPBIC.