MDCK, clone L-9

Origin: dog, kidney of adult Cocker spaniel . S.H. Madin, N.B. Darby,

1958 (clone L-9 was obtained from the Centers for Disease

Control and Prevention (CDC), Atlanta, USA).

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  EMEM

serum -  BS 10 %

subculture procedure - cells detachment using 50 mg chymopsine

in 500 ml EDTA 0.04 %, split ratio 1 : 5

cryoconservation - growth medium, 30 % BS, 5 % DMSO, 1.0 - 1.5x106 cells/ml in ampule.

Viability after cryoconservation: 80 - 90 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species:   karyological analysis

Other properties:

virus susceptibility:  influenza

Applications: cell biology, virology, biotechnology (test systems preparation)

Collections: SPBII.