MDCC-MSB1

Origin: chicken, lymphoblastoma.

            Submitted from Fridrich Loeffler Institute, Germany.

Morphology:  lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum -   FBS 10%

subculture procedure - optimal population density 2.0105 cells/cm2

cryoconservation - growth medium,  10% DMSO, 5.0-6.0106 cells/ml in ampule

Viability after cryoconservation:     96% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Applications:  cell biology

Collections: SPBIC.