MDBK-VIEV

Origin: bovine, kidney, subline of MDBK (NBL-1)

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:-1:4,

cryoconservation - BME 45%, BS 45%, DMSO 10%, 2.0 -3.0 x106 cells/ml in ampule

Viability after cryoconservation:   70 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n=60, variability in the range between 40-75 chromosomes,

modal number of chromosomes 42.    

Other properties:

virus susceptibility: IBR; parainfluenza 3; BVD.

Applications: virology, biotechnology 

Collections: MWIEV