bovine, kidney, subline of MDBK (NBL-1)
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split
cryoconservation - BME 45%, BS 45%, DMSO 10%, 2.0 -3.0 x106 cells/ml in ampule
Viability after cryoconservation:
70 % (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
Karyology: 2n=60, variability in the range between 40-75 chromosomes,
modal number of chromosomes 42.
virus susceptibility: IBR; parainfluenza 3; BVD.