MDBK (NBL-1)

Origin: bovine, kidney.

            Proc.Soc.Exp.Biol.Med.1958. 98:574; J.Natl.Cancer Inst.1986. 76:87-93.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM, BME or EMEM

serum -   FBS 10%

other components -   NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:2 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0106 cells/ml in ampule

Viability after cryoconservation:    90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 60, variability in the range between 40-57 chromosomes,

modal number of chromosomes 51-53, number of markers - 11-14 (differential dye),

number of polyploid cells 2.0%.

Plating efficiency:    19% (ATCC)

Other properties:

virus susceptibility: - alphaviruses, vesicular stomatitis, IBR, BVD, bovine parvoviruses,

 bovine adenoviruses I and III, parainfluenza 3.

Applications:  virology.

Collections: ATCC CCL 22; ECACC 90050801; SPBIC; MWIIW.