mouse DBA/2, rhabdomyosarcoma induced by methylcholanthrene.
Cytology, Russ. 1988. 30: 726.
Mode of cultivation:
Conditions for cultivation:
serum - BS 10-15%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:1), split ratio 1:10 - 1:15
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
Karyology: 2n=40, variability in the range between 50-60 chromosomes,
modal number of chromosomes 53, number of markers - 2 (differential dye)
tumorigenic in syngeneic animals