MCH-82

Origin: mouse DBA/2, rhabdomyosarcoma induced by methylcholanthrene.

            Cytology, Russ. 1988. 30: 726.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   BS 10-15%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:1), split ratio 1:10 - 1:15

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n=40, variability in the range between 50-60 chromosomes,

modal number of chromosomes 53, number of markers - 2 (differential dye)

Tumorigenicity:  tumorigenic in syngeneic animals

Applications: tumorigenicity:

Collections: SPBIC.