mouse C3H, rhabdomyosarcoma induced by methylcholanthrene.
Cytology, Russ. 1970. 12: 798.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:1), split ratio 1:5 - 1:10
cryoconservation - growth medium, 10% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
91% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 40, variability in the range between 57-85 chromosomes,
77-78 chromosomes in 30% of cells, some cells have 1-3 microchromosomes.
tumorigenic in syngeneic animals