McCoy B

Origin: mouse, cells obtained from synovial fluid of human knee joint with arthritis

 (Z. Zellforsch. 1957, 47: 158), but later one of sublines proved to be of mouse origin.

Proc. Soc. Exp. Biol. Med. 1965, 118: 354.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  EMEM

serum -  BS 10 % or  FBS 10%

other components NEAA 1%, ( without NEAA 1% - SPBII )

subculture procedure - cells detachment using EDTA 0.04 %, split ratio 1 : 3

cryoconservation - growth medium, can add 30 % BS, 5 % DMSO, 1.0-1.5x106 cells/ml in ampule.

Viability after cryoconservation: 80 - 90 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species:   karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=40, variability in the range between 55-63 chromosomes,

modal number of chromosomes 58-60, number of markers - 1 small telocentric

chromosome, some cells have dicentric chromosomes (routine dye), number of polyploid cells 2.6%.

Other properties:

virus susceptibility:  vesicular stomatitis.

Susceptibility to chlamidia.

Applications: cell biology, virology

Collections: ATCC CRL 1696, ECACC 90010305, SPBII, SPBIC..