Origin: mouse, cells obtained from synovial fluid of human knee joint with arthritis
(Z. Zellforsch. 1957, 47: 158), but later one of sublines
proved to be of mouse origin.
Proc. Soc. Exp. Biol. Med. 1965, 118: 354.
Mode of cultivation:
Conditions for cultivation:
medium - EMEM
serum - BS 10 % or FBS 10%
other components NEAA 1%, ( without NEAA 1% - SPBII )
subculture procedure - cells detachment using EDTA 0.04 %, split ratio 1 : 3
cryoconservation - growth medium, can add 30 % BS, 5 % DMSO, 1.0-1.5x106 cells/ml in ampule.
Viability after cryoconservation:
80 - 90 % (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=40, variability in the range between 55-63 chromosomes,
modal number of chromosomes 58-60, number of markers - 1 small telocentric
chromosome, some cells have dicentric chromosomes
(routine dye), number of polyploid cells 2.6%.
virus susceptibility: vesicular stomatitis.
Susceptibility to chlamidia.
cell biology, virology
ATCC CRL 1696, ECACC 90010305, SPBII, SPBIC..