McA RH 7777

Origin: rat Buffalo, hepatoma.

            Recent Results Cancer Res. 1974. 44: 103; źOnco-Developmental Gene Expression╗ NY,

Acad.Press. 1976: 259; J.Biol.Chem. 1982. 257: 5827.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using

EDTA 0.02% with 0.1 mg/ml chimopsin, split ratio 1:4-1:6,

optimal population density  0.5§105 cells/ml

cryoconservation -DMEM 70%, FBS 20%, glycerol 10%, 2.0-3.0§106 cells/ml in ampule

Viability after cryoconservation:     85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 62-82 chromosomes,

modal number of chromosomes 76 without markers (routine dye), number of polyploid cells 6.0%.

Other properties:

Production of a-fetoprotein, transitory cell antigen and albumin.

Susceptibility dexamethasone.

Applications: virology, tumorigenicity, cytotoxicity.

Collections: ATCC CRL 1601; ECACC 90021504; MWIIW.