MA-104

Origin: monkey, embryonic kidney of Macaca resus .

Appl. Microbiol. 1968, 16, 1770; J.Gen.Virol. 1979. 43: 513;

Arch.Virol. 1981. 70: 33; J.Clin.Microbiol. 1981. 13: 730.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation:   medium -  EMEM

serum -   BS or FBS 10 %

subculture procedure - cells detachment using EDTA 0.04 %, split ratio 1:3-1:5,

optimal population density 1.0-1.2x105 cells/ml

cryoconservation - EMEM 70%, FBS or BS 20%, glycerol 10%, 1.5-4.0x106 cells/ml in ampule.

Viability after cryoconservation:  80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species:   karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=42, variability in the range between 88-105 chromosomes,

modal number of chromosomes 100, number of markers - 1 large submetacentric

chromosome (routine dye). There are microchromosomes, number of polyploid cells 22.0%.

Other properties:

virus susceptibility:  alphaviruses, rubella, flaviviruses, vaccinia, RS, parainfluenza types 2 and 3, rotaviruses.

Applications: cell biology, virology, biotechnology (test systems preparation)

Collections: ECACC 85102918; MWIIW; SPBII.