LSM

Origin: mouse, connective, LS cells adapted to monolayer growth

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:3), split ratio 1:4, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     92% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species:  karyological, isoenzymological (LDH, G6PD) and immunofluorescent analysis

Karyology:   2n= 40, variability in the range between 52-58 chromosomes,

modal number of chromosomes 56, most cells have 1 metacentric with second

constriction (routine dye), number of polyploid cells 2%.

Tumorigenicity:  tumorigenic in syngenic animals

Applications:  oncology, biochemistry.

Collections:   SPBIC.