LS

Origin: mouse C3H/An, connective, derived from NCTC clone 929.

Proc.Roy.Soc. 1967. 168: 431-438.

Morphology:  round cells

Mode of cultivation: suspension

Conditions for cultivation: medium - EMEM or BME

serum -   FBS 10% or BS 10% (BME)

other components -   NEAA 1% (EMEM)

subculture procedure - optimal population density 0.8-1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0106 cells/ml in ampule

Viability after cryoconservation:     87% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH, G6PD)  and immunofluorescent analysis

Karyology:   2n= 40, variability in the range between 53-57 chromosomes,

modal number of chromosomes 55-56, number of polyploid cells 1%.

Applications:  biochemistry, cell biology.

Collections: SPBIC.