mouse C3H/An, connective, derived from NCTC clone 929.
Proc.Roy.Soc. 1967. 168: 431-438.
Mode of cultivation:
Conditions for cultivation:
medium - EMEM or BME
serum - FBS 10% or BS 10% (BME)
other components -
NEAA 1% (EMEM)
- optimal population density
cryoconservation - growth medium, 10% DMSO,
2.0õ106 cells/ml in ampule
Viability after cryoconservation:
87% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological, isoenzymological (LDH, G6PD) and
Karyology: 2n= 40, variability in the range between 53-57 chromosomes,
modal number of chromosomes 55-56, number of polyploid cells 1%.
biochemistry, cell biology.