chicken, ovarian lymphoma infected with Marek’s disease virus.
Mode of cultivation:
Conditions for cultivation:
medium - RPMI 1640
serum - FBS 10%
optimal population density 2.0-5.0ő105 cells/ml
cryoconservation - RPMI 1640 40%, FBS 50%, glycerol 10%, 5.0-8.0ő106 cells/ml in ampule
Viability after cryoconservation:
89% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 78, variability in the range between 5-44 large chromosomes,
modal number of large chromosomes 37, number of markers - 1 large submetacentric
chromosome (routine dye), the cells have many microchromosomes
(for example diploid karyotype - 78 chromosomes - includes 63 microchromosomes),
variability in the range between microchromosomes was not analysed.
virus susceptibility: alphaviruses, vesicular stomatitis.
Presence of particles of avian oncornaviruses C associated with destroied cells.