LOC

Origin: chicken, ovarian lymphoma infected with Marek’s disease virus.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum -   FBS 10%

subculture procedure - optimal population density 2.0-5.0ő105 cells/ml

cryoconservation - RPMI 1640 40%, FBS 50%, glycerol 10%, 5.0-8.0ő106 cells/ml in ampule

Viability after cryoconservation:     89% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 78, variability in the range between 5-44 large chromosomes,

modal number of large chromosomes 37, number of markers - 1 large submetacentric

chromosome (routine dye), the cells have many microchromosomes

(for example diploid karyotype - 78 chromosomes - includes 63 microchromosomes),

variability in the range between microchromosomes was not analysed.

Other properties:

virus susceptibility: alphaviruses, vesicular stomatitis.

Presence of particles of avian oncornaviruses C associated with destroied cells.

Applications: virology.

Collections: MWIIW.